Human Karyotyping (Biology) Lab Report Example | Topics and Well Written Essays - 1250 words

Human Karyotyping (Biology) - Lab Report ExampleAny deviation in the normal pattern could be govern out as a change that affects health and development of the individual. It is evident that human cells normally contain 23 pairs of chromosomes.Hypothesis If there is an increase or decrease in the number of chromosomes, the process is called aneuploidy it directly affects the development of the organism. These changes occur as a resolving power of non disjunction of chromosomes during the process of meiosis. As a result one of the daughter cells receives either extra or insufficient chromosomes.Variables The common form of aneuploidy is trisomy, which implies the presence of three copies of the chromosome in cells in place of normal devil copies. In Klinefelters Syndrome where the sex chromosome is present in extra copy resulting in XXY, where as if the trisomy occurs in autosome like in the case of cut out Syndrome trisomy of 21st chromosome occur and if this trisomy occur in 13th chromosome then it results in Trisomy 13 Syndrome resulting in cell with 47 chromosomes instead of usual 46.Methods Cut the homologous chromosomes. Arrange then on construction paper in descending order of their size. Glue them when arranged as in the figure A. any supernumerary chromosome is found match with its homologue to find out which chromosome is having additional copy. Arrange all the pairs of chromosomes with shorter end towards top and longer one towards the bottom. Mark them with the marker pen overlook out the genetic condition of the Karyotype.Raw data chromosomes condense during the process of cell division and are therefore visible under light microscope. In Karyotype cells are stained with Giemsa dye. Dark and light band pattern is observed.Metacentric chromosomes have centromere in the center or near the central leave of the chromosome. Acrocentric the centromere is near the end of the chromosome. Telocentric centromere is terminally located.Chromosomes